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mouse brain endothelial cell culture serum free media  (Celprogen Inc)


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    Celprogen Inc mouse brain endothelial cell culture serum free media
    Fig. 1 Schematic detailing mESC-D3 differentiation strategy to generate <t>brain-endothelial-like</t> cells and transwell BBB model in vitro. Schematic diagram of mESC-D3 directed monolayer differentiation protocol via initial mesodermal induction and subsequent endothelial cell differentiation and maturation. Representative phase contrast images illustrating morphological changes accompanying the various stages (Days) of differentiation
    Mouse Brain Endothelial Cell Culture Serum Free Media, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+brain+endothelial+cell+culture+serum+free+media/Mouse+Brain+Endothelial+Cell+Culture/pm37237379-43-15-23
    Average 91 stars, based on 1 article reviews
    mouse brain endothelial cell culture serum free media - by Bioz Stars, 2026-09
    91/100 stars

    Images

    1) Product Images from "Mouse embryonic stem cell-derived blood-brain barrier model: applicability to studying antibody triggered receptor mediated transcytosis."

    Article Title: Mouse embryonic stem cell-derived blood-brain barrier model: applicability to studying antibody triggered receptor mediated transcytosis.

    Journal: Fluids and barriers of the CNS

    doi: 10.1186/s12987-023-00437-0

    Fig. 1 Schematic detailing mESC-D3 differentiation strategy to generate brain-endothelial-like cells and transwell BBB model in vitro. Schematic diagram of mESC-D3 directed monolayer differentiation protocol via initial mesodermal induction and subsequent endothelial cell differentiation and maturation. Representative phase contrast images illustrating morphological changes accompanying the various stages (Days) of differentiation
    Figure Legend Snippet: Fig. 1 Schematic detailing mESC-D3 differentiation strategy to generate brain-endothelial-like cells and transwell BBB model in vitro. Schematic diagram of mESC-D3 directed monolayer differentiation protocol via initial mesodermal induction and subsequent endothelial cell differentiation and maturation. Representative phase contrast images illustrating morphological changes accompanying the various stages (Days) of differentiation

    Techniques Used: In Vitro, Cell Differentiation

    Fig. 2 Differentiation of mESC-D3 into mouse brain endothelial like-cells (mBECs). Flow cytometry analysis assessing transitional differentiation stages of endothelial differentiation. A Brachyury expression was assessed after 24 h of mesodermal induction with 5 ng/ml BMP4 and 30 ng/ ml VEGF and B endothelial progenitor Flk1 expression was assessed after 5 days in endothelial differentiation media. C–F GLUT1, CD31, VE-Cadherin and CLAUDIN 5 expression was assessed at the end of the differentiation period (Day 8). Red = unstained controls. G Temporal GLUT1 expression was assessed during the entire differentiation period. Increasing GLUT1 expression is shown with MFI (inset) at Day 1, 3, 5 and 7. The terminally differentiated mBECs exhibit cobblestone monolayer morphology and stained positive for key H endothelial (CD31, green) and (I–K) BBB-specific tight junction proteins (Claudin 5, Occludin and ZO-1; all green). Hoechst counterstain (blue); Scale bar = 20 µm. L In the presence of VEGF, the mBEC formed vascular–like structures in Matrigel assay within 24 h. Green = CFDA staining; Scale bar = 100 µm. M Following stimulation with inflammatory cytokines, the mBECs expressed immune adhesion molecule VCAM-1. Blue = unstained, Purple = non-stimulated; Orange = TNF-α and INF-γ stimulated. Representative images shown of 5 independent differentiations
    Figure Legend Snippet: Fig. 2 Differentiation of mESC-D3 into mouse brain endothelial like-cells (mBECs). Flow cytometry analysis assessing transitional differentiation stages of endothelial differentiation. A Brachyury expression was assessed after 24 h of mesodermal induction with 5 ng/ml BMP4 and 30 ng/ ml VEGF and B endothelial progenitor Flk1 expression was assessed after 5 days in endothelial differentiation media. C–F GLUT1, CD31, VE-Cadherin and CLAUDIN 5 expression was assessed at the end of the differentiation period (Day 8). Red = unstained controls. G Temporal GLUT1 expression was assessed during the entire differentiation period. Increasing GLUT1 expression is shown with MFI (inset) at Day 1, 3, 5 and 7. The terminally differentiated mBECs exhibit cobblestone monolayer morphology and stained positive for key H endothelial (CD31, green) and (I–K) BBB-specific tight junction proteins (Claudin 5, Occludin and ZO-1; all green). Hoechst counterstain (blue); Scale bar = 20 µm. L In the presence of VEGF, the mBEC formed vascular–like structures in Matrigel assay within 24 h. Green = CFDA staining; Scale bar = 100 µm. M Following stimulation with inflammatory cytokines, the mBECs expressed immune adhesion molecule VCAM-1. Blue = unstained, Purple = non-stimulated; Orange = TNF-α and INF-γ stimulated. Representative images shown of 5 independent differentiations

    Techniques Used: Flow Cytometry, Expressing, Staining, Matrigel Assay

    Fig. 4 Comparative RNASeq analysis of genes expression profiles in mBEC, bEnd.3 and pmBECs. Heatmaps depicting log2 transformed transcript abundances of A endothelial, B Wnt signaling, C tight junction D BBB receptor/transporters gene expression profiles in mBEC, immortalized bEnd.3 and primary mouse brain endothelial cells (pmBECs). Green = low expression; Red = high expression. Results are from three independent differentiations
    Figure Legend Snippet: Fig. 4 Comparative RNASeq analysis of genes expression profiles in mBEC, bEnd.3 and pmBECs. Heatmaps depicting log2 transformed transcript abundances of A endothelial, B Wnt signaling, C tight junction D BBB receptor/transporters gene expression profiles in mBEC, immortalized bEnd.3 and primary mouse brain endothelial cells (pmBECs). Green = low expression; Red = high expression. Results are from three independent differentiations

    Techniques Used: Expressing, Transformation Assay, Gene Expression

    Related Articles

    Cell Culture:

    Article Title: Mouse embryonic stem cell-derived blood-brain barrier model: applicability to studying antibody triggered receptor mediated transcytosis.
    Article Snippet: .. To induce endothelial differentiation and maturation, the medium was switched to complete Endothelial Media (EM): Mouse Brain Endothelial Cell Culture Serum Free Media (Celprogen, Torrance, California), 5% Fetal Bovine Serum (Hyclone, Logan, Utah), 5 ng/ ml bFGF (Thermo Fisher Scientific, Waltham, Massachusetts) and 10 mM all-trans Retinoic Acid (RA; Sigma-Aldrich, St. Louis, Missouri) on day 6. .. After 2 days of culture in EM medium, the cells were dissociated with 0.05% Trypsin–EDTA (Wisent, St-Bruno, QC, Canada) and filtered through a 40 μm sieve to eliminate residual basement membrane and endothelial cell clusters. mBEC were plated at a density of 7.5 × 105 cells/ cm2 or 1 × 106 cells/cm2 onto Collagen IV (80 μg/ml, Sigma-Aldrich, St. Louis, Missouri) and Fibronectin (20 μg/ml; Sigma-Aldrich, St. Louis, Missouri), or Laminin 521(10 μg/ml; Stem Cell Technologies, Vancouver, British Columbia), or Laminin 211(10 μg/ml; Biolamina, Sundbyberg, Sweden) or Laminin 511 (10 μg/ ml; AMSBIO, Abingdon, UK) coated Transwell inserts (1.12 cm2 cell growth area with 1 μm pore size; Corning, Canton, New York) in complete EM media containing 10 μM RA and 5 ng/ml bFGF.



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    mouse brain endothelial cell culture serum free media  (Celprogen Inc)
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    Celprogen Inc mouse brain endothelial cell culture serum free media
    Fig. 1 Schematic detailing mESC-D3 differentiation strategy to generate <t>brain-endothelial-like</t> cells and transwell BBB model in vitro. Schematic diagram of mESC-D3 directed monolayer differentiation protocol via initial mesodermal induction and subsequent endothelial cell differentiation and maturation. Representative phase contrast images illustrating morphological changes accompanying the various stages (Days) of differentiation
    Mouse Brain Endothelial Cell Culture Serum Free Media, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+brain+endothelial+cell+culture+serum+free+media/Mouse+Brain+Endothelial+Cell+Culture/pm37237379-43-15-23
    Average 91 stars, based on 1 article reviews
    mouse brain endothelial cell culture serum free media - by Bioz Stars, 2026-09
    91/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 1 Schematic detailing mESC-D3 differentiation strategy to generate brain-endothelial-like cells and transwell BBB model in vitro. Schematic diagram of mESC-D3 directed monolayer differentiation protocol via initial mesodermal induction and subsequent endothelial cell differentiation and maturation. Representative phase contrast images illustrating morphological changes accompanying the various stages (Days) of differentiation

    Journal: Fluids and barriers of the CNS

    Article Title: Mouse embryonic stem cell-derived blood-brain barrier model: applicability to studying antibody triggered receptor mediated transcytosis.

    doi: 10.1186/s12987-023-00437-0

    Figure Lengend Snippet: Fig. 1 Schematic detailing mESC-D3 differentiation strategy to generate brain-endothelial-like cells and transwell BBB model in vitro. Schematic diagram of mESC-D3 directed monolayer differentiation protocol via initial mesodermal induction and subsequent endothelial cell differentiation and maturation. Representative phase contrast images illustrating morphological changes accompanying the various stages (Days) of differentiation

    Article Snippet: To induce endothelial differentiation and maturation, the medium was switched to complete Endothelial Media (EM): Mouse Brain Endothelial Cell Culture Serum Free Media (Celprogen, Torrance, California), 5% Fetal Bovine Serum (Hyclone, Logan, Utah), 5 ng/ ml bFGF (Thermo Fisher Scientific, Waltham, Massachusetts) and 10 mM all-trans Retinoic Acid (RA; Sigma-Aldrich, St. Louis, Missouri) on day 6.

    Techniques: In Vitro, Cell Differentiation

    Fig. 2 Differentiation of mESC-D3 into mouse brain endothelial like-cells (mBECs). Flow cytometry analysis assessing transitional differentiation stages of endothelial differentiation. A Brachyury expression was assessed after 24 h of mesodermal induction with 5 ng/ml BMP4 and 30 ng/ ml VEGF and B endothelial progenitor Flk1 expression was assessed after 5 days in endothelial differentiation media. C–F GLUT1, CD31, VE-Cadherin and CLAUDIN 5 expression was assessed at the end of the differentiation period (Day 8). Red = unstained controls. G Temporal GLUT1 expression was assessed during the entire differentiation period. Increasing GLUT1 expression is shown with MFI (inset) at Day 1, 3, 5 and 7. The terminally differentiated mBECs exhibit cobblestone monolayer morphology and stained positive for key H endothelial (CD31, green) and (I–K) BBB-specific tight junction proteins (Claudin 5, Occludin and ZO-1; all green). Hoechst counterstain (blue); Scale bar = 20 µm. L In the presence of VEGF, the mBEC formed vascular–like structures in Matrigel assay within 24 h. Green = CFDA staining; Scale bar = 100 µm. M Following stimulation with inflammatory cytokines, the mBECs expressed immune adhesion molecule VCAM-1. Blue = unstained, Purple = non-stimulated; Orange = TNF-α and INF-γ stimulated. Representative images shown of 5 independent differentiations

    Journal: Fluids and barriers of the CNS

    Article Title: Mouse embryonic stem cell-derived blood-brain barrier model: applicability to studying antibody triggered receptor mediated transcytosis.

    doi: 10.1186/s12987-023-00437-0

    Figure Lengend Snippet: Fig. 2 Differentiation of mESC-D3 into mouse brain endothelial like-cells (mBECs). Flow cytometry analysis assessing transitional differentiation stages of endothelial differentiation. A Brachyury expression was assessed after 24 h of mesodermal induction with 5 ng/ml BMP4 and 30 ng/ ml VEGF and B endothelial progenitor Flk1 expression was assessed after 5 days in endothelial differentiation media. C–F GLUT1, CD31, VE-Cadherin and CLAUDIN 5 expression was assessed at the end of the differentiation period (Day 8). Red = unstained controls. G Temporal GLUT1 expression was assessed during the entire differentiation period. Increasing GLUT1 expression is shown with MFI (inset) at Day 1, 3, 5 and 7. The terminally differentiated mBECs exhibit cobblestone monolayer morphology and stained positive for key H endothelial (CD31, green) and (I–K) BBB-specific tight junction proteins (Claudin 5, Occludin and ZO-1; all green). Hoechst counterstain (blue); Scale bar = 20 µm. L In the presence of VEGF, the mBEC formed vascular–like structures in Matrigel assay within 24 h. Green = CFDA staining; Scale bar = 100 µm. M Following stimulation with inflammatory cytokines, the mBECs expressed immune adhesion molecule VCAM-1. Blue = unstained, Purple = non-stimulated; Orange = TNF-α and INF-γ stimulated. Representative images shown of 5 independent differentiations

    Article Snippet: To induce endothelial differentiation and maturation, the medium was switched to complete Endothelial Media (EM): Mouse Brain Endothelial Cell Culture Serum Free Media (Celprogen, Torrance, California), 5% Fetal Bovine Serum (Hyclone, Logan, Utah), 5 ng/ ml bFGF (Thermo Fisher Scientific, Waltham, Massachusetts) and 10 mM all-trans Retinoic Acid (RA; Sigma-Aldrich, St. Louis, Missouri) on day 6.

    Techniques: Flow Cytometry, Expressing, Staining, Matrigel Assay

    Fig. 4 Comparative RNASeq analysis of genes expression profiles in mBEC, bEnd.3 and pmBECs. Heatmaps depicting log2 transformed transcript abundances of A endothelial, B Wnt signaling, C tight junction D BBB receptor/transporters gene expression profiles in mBEC, immortalized bEnd.3 and primary mouse brain endothelial cells (pmBECs). Green = low expression; Red = high expression. Results are from three independent differentiations

    Journal: Fluids and barriers of the CNS

    Article Title: Mouse embryonic stem cell-derived blood-brain barrier model: applicability to studying antibody triggered receptor mediated transcytosis.

    doi: 10.1186/s12987-023-00437-0

    Figure Lengend Snippet: Fig. 4 Comparative RNASeq analysis of genes expression profiles in mBEC, bEnd.3 and pmBECs. Heatmaps depicting log2 transformed transcript abundances of A endothelial, B Wnt signaling, C tight junction D BBB receptor/transporters gene expression profiles in mBEC, immortalized bEnd.3 and primary mouse brain endothelial cells (pmBECs). Green = low expression; Red = high expression. Results are from three independent differentiations

    Article Snippet: To induce endothelial differentiation and maturation, the medium was switched to complete Endothelial Media (EM): Mouse Brain Endothelial Cell Culture Serum Free Media (Celprogen, Torrance, California), 5% Fetal Bovine Serum (Hyclone, Logan, Utah), 5 ng/ ml bFGF (Thermo Fisher Scientific, Waltham, Massachusetts) and 10 mM all-trans Retinoic Acid (RA; Sigma-Aldrich, St. Louis, Missouri) on day 6.

    Techniques: Expressing, Transformation Assay, Gene Expression